This barrio was so named after an image of the Infant Jesus that was found upon digging up a tree that could not be cut down using implements that people used. It was founded in 1854 and its original settlers were the Litan, Macatangay, Manalo and Patulot families. During the Spanish era, this barrio was frequently raided by tulisanes or brigands. During the Fil-American War, womenfolk of the barrio were abused by the Macabebe Scouts, who were working with soldiers of the US Army. In World War II, Santo Niño was an evacuation center for people from other towns, particularly Lipa.
Santo Niño is situated at approximately 13.8577, 121.1366, in the island of Luzon. Elevation at these coordinates is estimated at 178.7 meters or 586.3 feet above mean sea level.